Journal: EMBO Reports

Article Title: A gradient green-beard gene in fission yeast

doi: 10.1038/s44319-026-00748-x

Figure Lengend Snippet: ( A ) The architecture of Gsf2 proteins from the WT strain and the reference genomes. The number indicates the length of Gsf2 proteins. Four repeat motifs are shown. ( B ) The phenotypes of the WT cells after 2 h, 4 h, and 8 h with and without 0.1% acetic acid treatment. A representative image from three independent biological replicates is shown for each time point and condition. The other replicates were provided in Appendix Fig. . ( C ) Relative expression levels of gsf2 , measured by quantitative RT-PCR (qPCR), following treatment with or without 0.1% acetic acid for 2, 4, and 8 h. Error bars represent the mean ± SD of three biological replicates. Significance was determined by Student’s t-test. ( D , E ) Schematic diagram for constructing gsf2 knockout strain gsf2 Δ and gsf2 inducible expression strain gsf2IE . ( F ) The phenotypes of the yeast strains WT, gsf2IE , and gsf2 Δ under different culture condition, including EMM media, YE media, and YE media with 0.1% acetate acid. A representative image from three independent biological replicates is shown for each condition. The other replicates were provided in Appendix Fig. . ( G ) Relative expression levels of gsf2 in the yeast strains WT, gsf2IE , and gsf2 Δ cultured in EMM media, YE media, or YE media with 0.1% acetic acid. Error bars represent the mean ± SD of three biological replicates. Significance was determined by Student’s t-test. .

Article Snippet: ChamQTM SYBR qPCR Master Mix , Vazyme , cat#Q331-02.

Techniques: Expressing, Quantitative RT-PCR, Knock-Out, Cell Culture